Figure 1: Western Blot - Anti-HDAC3 Antibody (A90101)
Western blot analysis of extracts of various cell lines, using Anti-HDAC3 Antibody (A90101) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Figure 2: Western Blot - Anti-HDAC3 Antibody (A90101)
Western blot analysis of extracts of mouse brain, using Anti-HDAC3 Antibody (A90101) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-HDAC3 Antibody (A90101) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-HDAC3 Antibody (A90101) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-HDAC3 Antibody (A90101) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-HDAC3 Antibody (A90101) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using Anti-HDAC3 Antibody (A90101) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
![Flow Cytometry - Anti-HDAC3 Antibody [PCRP-HDAC3-3C9] (A277839) - Antibodies.com](https://cdn.antibodies.com/image/catalog/277/A277839_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-HDAC3 Antibody [PCRP-HDAC3-3C9] - BSA and Azide free (A278427) - Antibodies.com](https://cdn.antibodies.com/image/catalog/278/A278427_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-HDAC3 Antibody [PCRP-HDAC3-2D4] (A277838) - Antibodies.com](https://cdn.antibodies.com/image/catalog/277/A277838_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-HDAC3 Antibody [PCRP-HDAC3-2D4] - BSA and Azide free (A278426) - Antibodies.com](https://cdn.antibodies.com/image/catalog/278/A278426_1.jpg?profile=product_alternative)
![Western Blot - Anti-HDAC3 Antibody [ARC0016] (A305863) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305863_1.jpg?profile=product_alternative)
