Anti-HDAC2 Anticorps (A286069)

Name: Anti-HDAC2 Antibody
Brand: Antibodies.com
SKU: A286069
Price: 480.0 USD
Availability: InStock

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$480

Anticorps polyclonal de chèvre contre HDAC2 pour ELISA, WB, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-HDAC2 Antibody

Description du produit

Goat polyclonal antibody to HDAC2.

Applications

ELISA, WB, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:2,000, WB: 1-2µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human, Mouse

Réactivité croisée

This antibody may cross react with HDAC1

Immunogène

Synthetic peptide corresponding to the internal region of mouse HDAC2.

Séquence

C-PEDAVHEDSGDE

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

55-60 kDa

MW prédit

55.3kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

D10Wsu179e, HD 2, HD2, HDAC 2, HDAC2_HUMAN, Histone deacetylase 2, Histone deacetylase 2 (HD2), OTTHUMP00000017046, OTTHUMP00000227077, OTTHUMP00000227078, RPD3, transcriptional regulator homolog RPD3, YAF1, YY1 associated factor 1, YY1 transcription factor binding protein, Yy1bp

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-HDAC2 Antibody (A286069)

HDAC2 expression in HEK293 nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-HDAC2 Antibody (A286069) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-HDAC2 Antibody (A286069)

HDAC2 expression in U251 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-HDAC2 Antibody (A286069) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-HDAC2 Antibody (A286069)

HDAC2 expression in NIH3T3 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-HDAC2 Antibody (A286069) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic, plasma membrane, and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-HDAC2 Antibody (A286069)

HDAC2 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-HDAC2 Antibody (A286069) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.