Figure 1: Western Blot - Anti-HDAC1 Antibody (A84192)
HDAC1 expression in Human Duodenum (A) and Lymph Node (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-HDAC1 Antibody (A84192) at 0.1µg/ml and detected by chemiluminescence.
Figure 2: Western Blot - Anti-HDAC1 Antibody (A84192)
HDAC1 expression in HeLa cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-HDAC1 Antibody (A84192) at 0.5µg/ml and detected by chemiluminescence.
Figure 3: Western Blot - Anti-HDAC1 Antibody (A84192)
HDAC1 expression in NIH3T3 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-HDAC1 Antibody (A84192) at 0.1µg/ml and detected by chemiluminescence.
HDAC1 expression in NIH3T3 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-HDAC1 Antibody (A84192) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
HDAC1 enrichment at the p21 locus of 1µg MCF7 cell chromatin analyzed by ChIP. ChIP was performed using the Chromatrap® spin column kit (Protein G) and Anti-HDAC1 Antibody (A84192) at 2µg.
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