Rabbit polyclonal antibody to GSK3a (Phospho-Ser21)
Spécificité
The antibody detects endogenous level of GSK3a only when phosphorylated at serine 21and does not detect GSK-3b when phosphorylated at Ser9.
Applications
WB, IHC, IF
Recommander des dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivité
Human, Mouse, Rat
Immunogène
Peptide sequence around phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3a.
Hôte
Rabbit
Clonalité
Polyclonal
Conjuguer
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
MW prédit
51kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Stockage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3a (Phospho-Ser21) Antibody #11007 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from 293 cells, treated with insulin or calf intestinal phosphatase (CIP), using GSK3α (Phospho-Ser21) Antibody #11007.
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