Figure 1: Western Blot - Anti-GSK3 beta Antibody (A304928)
Western blot analysis of mouse brain showing detection of ~46.7 kDa GSK3 beta protein using Anti-GSK3 beta Antibody (A304928) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~46.7 kDa.
Immunohistochemistry analysis of human breast cancer, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-GSK3 beta Antibody (A304928) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
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