Figure 1: Western Blot - Anti-GSK3 alpha Antibody [ARC0462] (A307767)
Western blot analysis of extracts from wild type (WT) and GSK3alpha knockout (KO) HeLa cells, using Anti-GSK3 alpha Antibody [ARC0462] (A307767) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Figure 2: Western Blot - Anti-GSK3 alpha Antibody [ARC0462] (A307767)
Western blot analysis of extracts of Rat lung, using Anti-GSK3 alpha Antibody [ARC0462] (A307767) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Figure 3: Western Blot - Anti-GSK3 alpha Antibody [ARC0462] (A307767)
Western blot analysis of extracts of Mouse brain, using Anti-GSK3 alpha Antibody [ARC0462] (A307767) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 4: Western Blot - Anti-GSK3 alpha Antibody [ARC0462] (A307767)
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-GSK3 alpha Antibody [ARC0462] (A307767). This Western blot was performed on the immunoprecipitate using Anti-GSK3 alpha Antibody [ARC0462] (A307767) at a dilution of 1:1000.
Produits alternatifs à Anti-GSK3 alpha Anticorps [ARC0462] (A307767)