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Anti-GRP78 Anticorps (A305100)

Western Blot - Anti-GRP78 Antibody (A305100) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-GRP78 Antibody (A305100) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-GRP78 Antibody (A305100) - Antibodies.com Western Blot - Anti-GRP78 Antibody (A305100) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-GRP78 Antibody (A305100) - Antibodies.com
$520
Product Datasheet

Anticorps polyclonal de lapin contre GRP78 pour WB, IHC, ICC/IF et IP.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-GRP78 Antibody
Description du produit
Rabbit polyclonal antibody to GRP78.
Applications
WB, IHC, ICC/IF, IP
Recommander des dilutions
WB: 1:1,000, ICC/IF: 1:100
Reactivité
Human, Mouse, Rat, Bovine, Fungi, Hamster, Monkey, Rabbit, Xenopus, Canine
Immunogène
Rat GRP78 (Bip) synthetic peptide conjugated to KLH.
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Peptide affinity purification.
Concentration
1 mg/ml
Masse moléculaire
~78 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
78 kDa glucose regulated protein, 78 kDa glucose-regulated protein, AL022860, AU019543, BIP, D2Wsu141e, D2Wsu17e, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Endoplasmic reticulum lumenal Ca2+ binding protein grp78, Epididymis secretory sperm binding protein Li 89n, FLJ26106, Glucose Regulated Protein 78kDa, GRP 78, GRP-78, GRP78 BiP, GRP78_HUMAN, Heat shock 70 kDa protein 5, Heat Shock 70kDa Protein 5, Heat shock protein family A (Hsp70) member 5, HEL S 89n, Hsce70, HSPA 5, HSPA5, Immunoglobulin Heavy Chain Binding Protein, Immunoglobulin heavy chain-binding protein, mBiP, MIF2, Sez7
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-GRP78 Antibody (A305100) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-GRP78 Antibody (A305100)
Western blot analysis of rat tissue lysates showing detection of GRP78 protein using Anti-GRP78 Antibody (A305100) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence - Anti-GRP78 Antibody (A305100) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunofluorescence - Anti-GRP78 Antibody (A305100)
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GRP78 Antibody (A305100), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.
Immunocytochemistry/Immunofluorescence - Anti-GRP78 Antibody (A305100) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-GRP78 Antibody (A305100)
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GRP78 Antibody (A305100), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.
Western Blot - Anti-GRP78 Antibody (A305100) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - Anti-GRP78 Antibody (A305100)
Western blot analysis of Human, Dog, mouse Cell line lysates showing detection of GRP78 protein using Anti-GRP78 Antibody (A305100) at 1:1,000.

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