Anti-GRP78 Anticorps [3C5-1A4] (A305024)

Name: Anti-GRP78 Antibody [3C5-1A4]
Brand: Antibodies.com
SKU: A305024
Price: 490.0 USD
Availability: InStock

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$490

Anticorps monoclonal [3C5-1A4] de souris contre GRP78 pour WB, ICC/IF et ELISA.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

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Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Mots clés :

Ribosomal Marker

Spécifications

Nom du produit

Anti-GRP78 Antibody [3C5-1A4]

Description du produit

Mouse monoclonal [3C5-1A4] antibody to GRP78.

Applications

WB, ICC/IF, ELISA

Recommander des dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivité

Human, Mouse, Rat

Immunogène

Full-length recombinant rat GRP78.

Hôte

Mouse

Clonalité

Monoclonal

Clone

3C5-1A4

Isotype

IgG1

Chaînes légères

kappa

Conjuguer

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Masse moléculaire

~78 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

78 kDa glucose regulated protein, 78 kDa glucose-regulated protein, AL022860, AU019543, BIP, D2Wsu141e, D2Wsu17e, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Endoplasmic reticulum lumenal Ca2+ binding protein grp78, Epididymis secretory sperm binding protein Li 89n, FLJ26106, Glucose Regulated Protein 78kDa, GRP 78, GRP-78, GRP78 BiP, GRP78_HUMAN, Heat shock 70 kDa protein 5, Heat Shock 70kDa Protein 5, Heat shock protein family A (Hsp70) member 5, HEL S 89n, Hsce70, HSPA 5, HSPA5, Immunoglobulin Heavy Chain Binding Protein, Immunoglobulin heavy chain-binding protein, mBiP, MIF2, Sez7

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (2)

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Figure 1: Western Blot - Anti-GRP78 Antibody [3C5-1A4] (A305024)

Western blot analysis of Human, mouse, rat NIH3T3, rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Anti-GRP78 Antibody [3C5-1A4] (A305024) at 1:1,000 for 1 hour at room temperature. Lane 1: MW ladder. Lane 2: mouse NIH3T3. Lane 3: rat Brain. Lane 4: human HEK-293. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was HRP Goat Anti-Mouse at 1:50 for 1 hour at room temperature. Color Development: TMB solution for 5 minutes at room temperature. Predicted/Observed Size: ~78 kDa.

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Figure 2: Immunofluorescence - Anti-GRP78 Antibody [3C5-1A4] (A305024)

Immunocytochemistry/Immunofluorescence analysis of mouse fibroblast cell line (NIH 3T3), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-GRP78 Antibody [3C5-1A4] (A305024), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Nucleus. Magnification: 60X.