Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Stockage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Notes générales
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonymes
G protein coupled receptor family C group 5 member D, G-protein coupled receptor family C group 5 member D, GPC5D_HUMAN, GPRC5D
ELISA plates were pre-coated with Recombinant Human GPCR GPRC5D Protein (Fc Chimera 6xHis Tag) (A318347) at 2 µg/ml (100 µl/well) which can bind Anti-GPCR GPRC5D Antibody [DM61] - BSA and Azide free (A318636) in a linear range of 1-100 ng/ml.
Expi 293 cell line was transfected with irrelevant protein (A) and human GPRC5D (B) were surface stained with Anti-GPCR GPRC5D Antibody [DM61] - BSA and Azide free (A318636) at 15µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
Immunohistochemistry staining of H929 cells. A. With Anti-GPCR GPRC5D Antibody [DM61] - BSA and Azide free (A318636) at 5.9 ug/ml. B. With Anti-GPCR GPRC5D Antibody [DM90] - BSA and Azide free (A318616) at 8.8 ug/ml. Anti-GPCR GPRC5D Antibody [DM61] - BSA and Azide free (A318636) can specifically react with GPRC5D in IHC application.
Flow cytometry data of serially titrated Anti-GPCR GPRC5D Antibody [DM61] - BSA and Azide free (A318636) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different Rabbit Anti-GPRC5D Monoclonal Antibody clones by titration of different concentrations onto H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.