Recombinant Anti-Golgi Complex Anticorps [GLG1/7174R] (A316425)

Anti-Golgi Complex Antibody [GLG1/7174R] - BSA and Azide free

Recombinant rabbit monoclonal [GLG1/7174R] antibody to Golgi Complex.

This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.

IHC

HepG2, A431 or HeLa cells. Human placenta, tonsil or ovary.

IHC: (Frozen & Formalin-fixed) 0.5-1 µg/ml

Human

This antibody does not react with Mouse or Rat.

Recombinant full-length human GLG1 protein.

Rabbit

Monoclonal

IgG

kappa

Unconjugated

Protein A/G chromatography.

1 mg/ml

134 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

AI593353, AW537898, CFR 1, CFR-1, CFR1, Cys -rich FGF Receptor, Cysteine rich fibroblast growth factor receptor, Cysteine-rich fibroblast growth factor receptor, DKFZp686L08213, E selectin ligand 1, E-selectin ligand 1, ESL 1, ESL-1, ESL1, FLJ23319, FLJ23967, FLJ32797, GLG1, Golgi apparatus protein 1, golgi glycoprotein 1, Golgi sialoglycoprotein MG 160, Golgi sialoglycoprotein MG-160, GSLG1_HUMAN, MG 160, MG160, Mouse, homolog of, ESL1, Selel, Slectin, endothelial cell, ligand