This antibody recognises the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40 kDa forming a heterotrimeric protein complex with ß and ? subunits. Two isoforms of the a subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2.Rabbit anti Mouse G Protein alpha 0 antibody has been used successfully for the detection of G(o) in Drosophila by western blotting.

Applications

WB, ELISA

Recommander des dilutions

ELISA: 1:500 - 1:1,000

Reactivité

Mouse, Drosophila

Immunogène

Synthetic peptide sequence C-ANNLRGCGLY corresponding to amino acids 345 - 354 of the native isoform 1 molecule conjugated to Keyhole Limpet Haemocyanin.

Séquence

C-ANNLRGCGLY

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Protein G affinity chromatography.

Concentration

5 mg/ml

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Stockage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Notes générales

Rabbit anti Mouse G Protein alpha 0 antibody recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40 kDa forming a heterotrimeric protein complex with ß and ? subunits. Two isoforms of the a subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2.Rabbit anti Mouse G Protein alpha 0 antibody has been used successfully for the detection of G(o) in Drosophila by western blotting (Guo et al. 2011).

Synonymes

DKFZp686O0962, G alpha o, G protein alpha 0, G0 Protein alpha, Galphao, GNAO, GNAO 1, GNAO_HUMAN, Go alpha, Guanine nucleotide binding protein (G protein) alpha activating activity polypeptide O, Guanine nucleotide binding protein alpha activating polypeptide, Guanine nucleotide binding protein G(o) subunit alpha, Guanine nucleotide-binding protein G(o) subunit alpha

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.