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Anti-GATA3 Anticorps [ARC0115] (A307313)

Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313) - Antibodies.com Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313) - Antibodies.com Chromatin Immunoprecipitation - Anti-GATA3 Antibody [ARC0115] (A307313) - Antibodies.com
$470
Product Datasheet

Anticorps monoclonal [ARC0115] de lapin contre GATA3 pour WB, IP et ChIP.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-GATA3 Antibody [ARC0115]
Description du produit
Rabbit monoclonal [ARC0115] antibody to GATA3.
Applications
WB, IP, ChIP
Recommander des dilutions
WB: 1:500-1:2,000, IP: 1:100-1:500, ChIP: 1:100-1:500
Reactivité
Human, Mouse
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GATA3 (P23771).
Séquence
MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSH
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC0115
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
48 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
GATA 3, GATA binding factor 3, GATA binding protein 3, GATA-binding factor 3, GATA3_HUMAN, HDR, HDRS, MGC2346, MGC5199, MGC5445, Trans acting T cell specific transcription factor GATA 3, Trans-acting T-cell-specific transcription factor GATA-3
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313)
Western blot analysis of extracts of various cell lines, using Anti-GATA3 Antibody [ARC0115] (A307313) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313)
Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-GATA3 Antibody [ARC0115] (A307313). This Western blot was performed on the immunoprecipitate using Anti-GATA3 Antibody [ARC0115] (A307313) at a dilution of 1:500.
Chromatin Immunoprecipitation - Anti-GATA3 Antibody [ARC0115] (A307313) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Chromatin Immunoprecipitation - Anti-GATA3 Antibody [ARC0115] (A307313)
Chromatin immunoprecipitation (ChIP) analysis of extracts of MCF7 cells, using Anti-GATA3 Antibody [ARC0115] (A307313) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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