Anti-GAPDH Anticorps (A85377)

Name: Anti-GAPDH Antibody
Brand: Antibodies.com
SKU: A85377
Price: 185.0 USD
Availability: InStock

Ce produit est identique à Novus (NB300-327). Découvrez plus pour moins cher.

Cliquez pour ouvrir le point de vue élargi

$185

Anticorps polyclonal de lapin contre GAPDH pour WB, ICC/IF et IHC.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Cytoplasm Marker Cytoskeleton Marker Loading Control Nuclear Marker

Spécifications

Nom du produit

Anti-GAPDH Antibody

Description du produit

Rabbit polyclonal antibody to GAPDH.

Applications

WB, ICC/IF, IHC

Recommander des dilutions

WB: 1:30,000, ICC/IF: 1:2,000, IHC: 1:2,000

Reactivité

Human, Horse, Bovine, Porcine, Chicken, Rat, Mouse

Immunogène

Recombinant full-length human GAPDH, expressed in and purified from E. coli.

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Masse moléculaire

36 kDa

Pureté

Whole antiserum.

Forme du produit

Liquid

Formulation

Supplied as an aliquot of serum with 5mM Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

38 kDa BFA-dependent ADP-ribosylation substrate, aging associated gene 9 protein, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, Epididymis secretory sperm binding protein Li 162eP, G3PD, G3PDH, G3P_HUMAN, GAPD, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL S 162eP, HEL-S-162eP, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38 component, OCT1 coactivator in S phase, 38-KD component, peptidyl cysteine S nitrosylase GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, wu:fb33a10

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Immunofluorescence - Anti-GAPDH Antibody (A85377)

HeLa cells stained with Anti-GAPDH Antibody (red), Anti-TAF15 Antibody (A85450 | green) and DNA (blue). The Anti-GAPDH Antibody reveals strong cytoplasmic staining, while the Anti-TAF15 Antibody reveals a granular nuclear localization.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Immunofluorescence - Anti-GAPDH Antibody (A85377)

Immunofluorescent analysis of HeLa cells stained with Anti-GAPDH Antibody (A85377), at a dilution of 1:2,000 in red. Blue is Hoechst staining of nuclear DNA. The Anti-GAPDH Antibody (A85377) produces diffuse cytoplasmic staining of cells.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Western Blot - Anti-GAPDH Antibody (A85377)

Western blot analysis of different cell cytosolic or nuclear enriched fractions, using Anti-GAPDH Antibody (A85377), at a dilution of 1:20,000, in red. The lanes contain samples of: [1] Protein standards, in red, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions. The same blot was simultaneously probed with Anti-SF3B4 Antibody (A85417), at a dilution of 1:1,000, in green. In contrast to GAPDH, SF3B4 is exclusively expressed in the nuclear fraction.