Anti-GAD67 Anticorps (A82616)

Name: Anti-GAD67 Antibody
Brand: Antibodies.com
SKU: A82616
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Novus (NBP1-06989). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre GAD67 pour ELISA, WB, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-GAD67 Antibody

Description du produit

Goat polyclonal antibody to GAD67.

Applications

ELISA, WB, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:128,000, WB: 0.3-1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human, Mouse, Rat

Réactivité croisée

This antibody is not expected to cross react with GAD2.

Immunogène

Synthetic peptide corresponding to the internal region of human GAD67.

Séquence

C-PDSPQRREKLHK

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

70 kDa

MW prédit

66.9kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

67 kDa glutamic acid decarboxylase, CPSQ1, DCE1, DCE1_HUMAN, EC 4.1.1.15, FLJ45882, GAD, GAD 67, GAD-67, GAD1, Glutamate decarboxylase 1, Glutamate decarboxylase 1 (brain, 67kDa), Glutamate decarboxylase 1 brain 67kD, Glutamate decarboxylase 1 brain 67kDa, Glutamate decarboxylase 67 kDa isoform, Glutamate decarboxylase, brain, 67-KD, OTTHUMP00000041055, SCP

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-GAD67 Antibody (A82616)

GAD67 expression in Human Cerebellum (A), Mouse Brain (B), and Rat Brain (C) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-GAD67 Antibody (A82616) at 1µg/ml (A-B) or 0.3µg/ml (C) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-GAD67 Antibody (A82616)

GAD67 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-GAD67 Antibody (A82616) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-GAD67 Antibody (A82616)

GAD67 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-GAD67 Antibody (A82616) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.