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GAB1 (phospho Tyr659) Cell Based Kit Elisa (A102305)

Nom du produit
GAB1 (phospho Tyr659) Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This GAB1 (phospho Tyr659) Cell Based ELISA Kit allows for the detection of GAB1 (phospho Tyr659) and the effects that certain stimulation conditions have on GAB1 (phospho Tyr659) expression in different cell lines. Qualitative determination of GAB1 (phospho Tyr659) concentration is achieved by an indirect ELISA format. In essence, the GAB1 (phospho Tyr659) is captured by Anti-GAB1 (phospho Tyr659) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this GAB1 (phospho Tyr659) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-GAB1 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
Gab 1, GAB1_HUMAN, GRB 2 associated binder 1, GRB 2 associated binding protein 1, GRB2 associated binding protein 1 isoform a, GRB2 associated binding protein 1 isoform b, GRB2-associated binder 1, GRB2-associated-binding protein 1, Growth factor receptor bound protein 2-associated protein 1
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - GAB1 (phospho Tyr659) Cell Based ELISA Kit (CBP1301) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - GAB1 (phospho Tyr659) Cell Based ELISA Kit (CBP1301)
Western blot analysis of extracts from NIH-3T3 cells treated with Insulin 0.01U/ml 15', using Anti-GAB1 (phopho Tyr659) Antibody.
Protocol Diagram - GAB1 (phospho Tyr659) Cell Based ELISA Kit (CBP1301) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: GAB1 (phospho Tyr659) Cell Based ELISA Kit (CBP1301)
A diagram showing the protocol for GAB1 (phospho Tyr659) Cell Based ELISA Kit.
Protocol Summary - GAB1 (phospho Tyr659) Cell Based ELISA Kit (CBP1301) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: GAB1 (phospho Tyr659) Cell Based ELISA Kit (CBP1301)
A summary of the protocol for GAB1 (phospho Tyr659) Cell Based ELISA Kit.
Western Blot - Control Antibody - GAB1 (phospho Tyr659) Cell Based ELISA Kit (CBP1301) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - GAB1 (phospho Tyr659) Cell Based ELISA Kit (CBP1301)
The mouse monoclonal antibody to GAPDH is used as a positive control inGAB1 (phospho Tyr659) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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