Synthetic peptide corresponding to the internal region of human FXR1.
Séquence
C-RIEGDNENKLPRED
Hôte
Goat
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Masse moléculaire
75+80 kDa
MW prédit
59.9kDa
Forme du produit
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonymes
Fragile X mental retardation autosomal homolog 1, Fragile X mental retardation syndrome related protein 1, Fragile X mental retardation syndrome-related protein 1, FXR1P, FXR1_HUMAN, hFXR1p
Figure 1: Western Blot - Anti-FXR1 Antibody (A83457)
FXR1 expression in NIH3T3 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-FXR1 Antibody (A83457) at 1µg/ml and detected by chemiluminescence.
FXR1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-FXR1 Antibody (A83457) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
FXR1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-FXR1 Antibody (A83457) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
FXR1 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-FXR1 Antibody (A83457) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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