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FOXL1 Cell Based Kit Elisa (A103696)

Nom du produit
FOXL1 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This FOXL1 Cell Based ELISA Kit allows for the detection of FOXL1 and the effects that certain stimulation conditions have on FOXL1 expression in different cell lines. Qualitative determination of FOXL1 concentration is achieved by an indirect ELISA format. In essence, the FOXL1 is captured by Anti-FOXL1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this FOXL1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
FKH 6, FKH6, FKHL 11, FKHL11, Forkhead (Drosophila) like 11, Forkhead box L1, Forkhead box protein L1, Forkhead related protein FKHL11, Forkhead related transcription factor 7, Forkhead-related protein FKHL11, Forkhead-related transcription factor 7, FOX L1, FOXL 1, FOXL1_HUMAN, FREAC 7, FREAC-7, FREAC7
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - FOXL1 Cell Based ELISA Kit (CB5978) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - FOXL1 Cell Based ELISA Kit (CB5978)
Western blot analysis of extracts from COS7/A549 cells, using Anti-FOXL1 Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - FOXL1 Cell Based ELISA Kit (CB5978) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: FOXL1 Cell Based ELISA Kit (CB5978)
A diagram showing the protocol for FOXL1 Cell Based ELISA Kit.
Protocol Summary - FOXL1 Cell Based ELISA Kit (CB5978) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: FOXL1 Cell Based ELISA Kit (CB5978)
A summary of the protocol for FOXL1 Cell Based ELISA Kit.
Western Blot - Control Antibody - FOXL1 Cell Based ELISA Kit (CB5978) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - FOXL1 Cell Based ELISA Kit (CB5978)
The mouse monoclonal antibody to GAPDH is used as a positive control inFOXL1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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