Mouse monoclonal [PCRP-FOXI1-1C4] antibody to FOXI1.
Specificity
FOXL1 is a 337 amino acid protein encoded by the mouse gene FOXL1. FOXL1 belongs to the forkhead family and contains one forkhead DNA-binding domain. The HNF3/forkhead family includes a large number of transcription factors that share a structurally related DNA binding domain. forkhead factors are known to play important roles both during development and in adults. FOXL1 is a winged helix transcriptional regulator expressed in the mesenchymal layer of developing and mature gastrointestinal tract. FOXL1-deficient mice exhibit various defects not only in the epithelial layer of the gastrointestinal tract but also in gut-associated lymphoid tissues. In the small intestine of FOXL1-deficient mice, the formation of Peyers patches is affected, particularly in the caudal region. FOXL1 is a mesenchymal modifier of the Adenomatous Polyposis Coli (APC) gene products and plays a key role in gastrointestinal tumorigenesis.
Applications
IP, Flow Cytometry, IF, WB, IHC-P
Positive Control
HeLa or U87 cells. Ubiquitous tissue expression.
Dilutions
IP: 1-2 µg per 100-500ug of total protein, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant fragment (around Amino Acids 104-258) of human FOXL1 protein. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
PCRP-FOXI1-1C4
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
36-41 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
