Western Blot - Anti-Flightless I Antibody (A88416)
Western blot analysis of extracts of various cell lines, using Anti-Flightless I Antibody (A88416) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5S.
Immunohistochemistry - Anti-Flightless I Antibody (A88416)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Flightless I Antibody (A88416) at a dilution of 1:100 (40x lens).