This antibody recognises human Fibroblast Growth Factor (FGF) basic, otherwise known as FGF 2 (fibroblast growth factor 2), a member of the heparin-binding growth factor family which exists in both cytosolic and nuclear isoforms, ranging in size from 18-24kDa, expressed by the majority of cells and tissues.FGF basic is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumour vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis. The recombinant basic FGF protein used as immunogen for development of Rabbit anti Human FGF basic polyclonal antibody corresponds to the C-terminal portion of the molecule (A135 - S288), present in all known isoforms of human FGF basic, thus all isoforms are expected to be recognized by this Rabbit anti Human FGF basic polyclonal antibody.

Applications

WB, ELISA, IHC-P, Functional Assays

Recommander des dilutions

ELISA: 0.5 µg/ml - 2.0 µg/ml, Functional Assays: 0.25 - 0.40 µg/ml, IHC-P: 0.25 - 0.5 µg/ml, WB: 0.1 - 0.2 µg/ml

Reactivité

Human

Immunogène

Recombinant human FGF basic.

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Concentration

1 mg/ml after reconstitution.

Forme du produit

Lyophilized

Reconstitution

Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add sodium azide.

Formulation

Supplied in Phosphate Buffered Saline.

Stockage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Notes générales

Rabbit anti Human FGF basic polyclonal antibody recognizes human Fibroblast Growth Factor (FGF) basic, otherwise known as FGF 2 (fibroblast growth factor 2), a member of the heparin-binding growth factor family which exists in both cytosolic and nuclear isoforms, ranging in size from 18-24kDa, expressed by the majority of cells and tissues.FGF basic is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumour vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis. The recombinant basic FGF protein used as immunogen for development of Rabbit anti Human FGF basic polyclonal antibody corresponds to the C-terminal portion of the molecule (A135 - S288), present in all known isoforms of human FGF basic, thus all isoforms are expected to be recognized by this Rabbit anti Human FGF basic polyclonal antibody.

Synonymes

Basic fibroblast growth factor, Basic fibroblast growth factor bFGF, BFGF, FGF 2, FGF B, FGF-2, FGF2 basic, FGF2_HUMAN, FGFB, Fibroblast growth factor 2, Fibroblast growth factor 2 (basic), Fibroblast growth factor, basic, HBGF 2, HBGF-2, HBGF2, HBGH 2, HBGH2, Heparin binding growth factor 2 precursor, Heparin-binding growth factor 2, Prostatropin

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.