Rabbit polyclonal antibody to FER (Phospho-Tyr402)
Specificity
The antibody detects endogenous levels of FER only when phosphorylated at tyrosine 402.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of tyrosine 402(V-N-Y(p)-E-E) derived from Human FER .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from JK cells (Lane 2) and COS7 cells (Lane 3), using FER (Phospho-Tyr402) Antibody #11730. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using FER (Phospho-Tyr402) antibody #11730 (left)or the same antibody preincubated with blocking peptide (right).
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