Anti-FEN1 Antibody (A283940)

Anti-FEN1 Antibody

Rabbit polyclonal antibody to FEN1.

This antibody recognises human Flap endonuclease 1 also known as Flap structure-specific endonuclease, FEN1, Maturation factor 1 or DNase IV. FEN1 is a 380 amino acid ~45 kDa member of the FEN1 sub family of XPG/RAD2 endonucleases which has an important role in lagging strand DNA synthesis and base excision repair. It is hypothesised that FEN1 may play a role in the inhibltion of triplet repeat expansion. Two isoforms of FEN1 are generated by alternative initiation, isoform 1 against which rabbit anti human FEN1 antibody is generated and a truncated isoform FENMIT, lacking the N-terminal region and having a mitochondrial distribution, FENMIT also lacks DNAase activity. Initial results suggest that rabbit anti human FEN1 antibody does not recognize the truncated form of human FEN1.Fen1 is expressed in the nucleus of a wide variety of organ strusctures notably in those of the hematopoietic system including spleen, lymph nodes and spleen. recognises a band of ~45 kDa in whole cell lysates of HeLa and 293T by western blotting and has successfully been used for immunoprecipitation of FEN1 from HeLa cell lysates.

WB, IP

IP: 2ug/mg protein - 10ug/mg protein, WB: 1:2,000 - 1:10,000, AHP837

Human, Mouse

Synthetic peptide corresponding to a region within the N terminal domain of human FEN1 (locus link ID 2237).

Rabbit

Polyclonal

IgG

Approximately 42kD in HeLa cell lysates.

Liquid

Supplied in TRIS-Citrate/Phosphate Buffered Saline with 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.

Rabbit anti Human FEN1 antibody recognizes human Flap endonuclease 1 also known as Flap structure-specific endonuclease, FEN1, Maturation factor 1 or DNase IV. FEN1 is a 380 amino acid ~45 kDa member of the FEN1 sub family of XPG/RAD2 endonucleases which has an important role in lagging strand DNA synthesis and base excision repair (Kim et al. 1998). It is hypothesised that FEN1 may play a role in the inhibltion of triplet repeat expansion (Vallur and Maizels 2010).Two isoforms of FEN1 are generated by alternative initiation, isoform 1 against which rabbit anti human FEN1 antibody is generated and a truncated isoform FENMIT, lacking the N-terminal region and having a mitochondrial distribution, FENMIT also lacks DNAase activity (Kazak et al. 2013). Initial results suggest that rabbit anti human FEN1 antibody does not recognize the truncated form of human FEN1.Fen1 is expressed in the nucleus of a wide variety of organ strusctures notably in those of the hematopoietic system including spleen, lymph nodes and spleen. Rabbit anti human FEN1 antibody recognizes a band of ~45 kDa in whole cell lysates of HeLa and 293T by western blotting and has successfully been used for immunoprecipitation of FEN1 from HeLa cell lysates.

DNase IV, FEN-1, Flap endonuclease 1, Flap structure-specific endonuclease 1, hFEN-1, Maturation factor 1, MF1, RAD2