Figure 1: Western Blot - Anti-FEN1 Antibody (A13351)
Western blot analysis of extracts of various cell lines, using Anti-FEN1 Antibody (A13351) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-FEN1 Antibody (A13351) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-FEN1 Antibody (A13351) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 6: Western Blot - Anti-FEN1 Antibody (A13351)
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 1µg of Anti-FEN1 Antibody (A13351). This Western blot was performed on the immunoprecipitate using Anti-FEN1 Antibody (A13351) at a dilution of 1:1000.