Anti-Fatty Acid Synthase Anticorps (A12631)

Name: Anti-Fatty Acid Synthase Antibody
Brand: Antibodies.com
SKU: A12631

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Ce produit est discontinué

Anti-Fatty Acid Synthase Anticorps (A12631) a été abandonné et n'est plus disponible.

Voir toutes Anti-Fatty Acid Synthase Anticorps.

Spécifications

Nom du produit

Anti-Fatty Acid Synthase Antibody

Description du produit

Rabbit polyclonal antibody to Fatty Acid Synthase.

Applications

WB, IHC, ICC/IF, IP

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 2221-2511 of human Fatty Acid Synthase (FASN) (NP_004095.4).

Séquence

SLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

273 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-oxoacyl-[acyl-carrier-protein] synthase, Enoyl-[acyl-carrier-protein] reductase, FAS, FASN, FAS_HUMAN, MGC14367, MGC15706, OA 519, Oleoyl-[acyl-carrier-protein] hydrolase, SDR27X1, Short chain dehydrogenase/reductase family 27X member 1, [Acyl-carrier-protein] S acetyltransferase, [Acyl-carrier-protein] S malonyltransferase

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-Fatty Acid Synthase Antibody (A12631)

Western blot analysis of extracts of various cell lines, using Anti-Fatty Acid Synthase Antibody (A12631) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-Fatty Acid Synthase Antibody (A12631)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Fatty Acid Synthase Antibody (A12631) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Fatty Acid Synthase Antibody (A12631)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Fatty Acid Synthase Antibody (A12631) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-Fatty Acid Synthase Antibody (A12631)

Immunofluorescence analysis of U2OS cells using Anti-Fatty Acid Synthase Antibody (A12631) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).