Western Blot - Anti-Estrogen Related Receptor alpha Antibody (A90033)
Western blot analysis of extracts of various cell lines, using Anti-Estrogen Related Receptor alpha Antibody (A90033) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Estrogen Related Receptor alpha Antibody (A90033)
Immunohistochemistry analysis of paraffin-embedded human uterus using Anti-Estrogen Related Receptor alpha Antibody (A90033) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Estrogen Related Receptor alpha Antibody (A90033)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Estrogen Related Receptor alpha Antibody (A90033) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Estrogen Related Receptor alpha Antibody (A90033)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Estrogen Related Receptor alpha Antibody (A90033) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Estrogen Related Receptor alpha Antibody (A90033)
Immunofluorescence analysis of rat colon using Anti-Estrogen Related Receptor alpha Antibody (A90033) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Estrogen Related Receptor alpha Antibody (A90033)
Immunofluorescence analysis of A-431 using Anti-Estrogen Related Receptor alpha Antibody (A90033) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).