Anti-Estrogen Receptor beta Anticorps (A83108)

Name: Anti-Estrogen Receptor beta Antibody
Brand: Antibodies.com
SKU: A83108
Price: 480.0 USD
Availability: InStock

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$480

Anticorps polyclonal de chèvre contre Estrogen Receptor beta pour ELISA, WB et IF.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-Estrogen Receptor beta Antibody

Description du produit

Goat polyclonal antibody to Estrogen Receptor beta.

Applications

ELISA, WB, IF

Recommander des dilutions

ELISA: 1:128,000, WB: 1-3µg/ml, IF: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region, amino acids 417-428, of human Estrogen Receptor beta.

Séquence

C-TQDADSSRKLAH

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

50 kDa

MW prédit

53.0kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

ER BETA, ER-beta, Erb, ESR B, ESR BETA, ESR2, ESR2_HUMAN, ESRB, ESTRB, estrogen nuclear receptor beta variant a, estrogen nuclear receptor beta variant b, Estrogen receptor 2, estrogen receptor 2 (ER beta), estrogen receptor beta 4, NR3A2, Nuclear receptor subfamily 3 group A member 2

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-Estrogen Receptor beta Antibody (A83108)

Estrogen Receptor beta expression in Human Placenta lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Estrogen Receptor beta Antibody (A83108) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-Estrogen Receptor beta Antibody (A83108)

Estrogen Receptor beta expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Estrogen Receptor beta Antibody (A83108) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-Estrogen Receptor beta Antibody (A83108)

Estrogen Receptor beta expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Estrogen Receptor beta Antibody (A83108) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Weak cytoplasmic and strong nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.