Anti-ERp57 Anticorps [4F9] (A304860)

Name: Anti-ERp57 Antibody [4F9]
Brand: Antibodies.com
SKU: A304860
Price: 500.0 USD
Availability: InStock

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$500

Anticorps monoclonal [4F9] de souris contre ERp57 pour WB et ICC/IF.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-ERp57 Antibody [4F9]

Description du produit

Mouse monoclonal [4F9] antibody to ERp57.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivité

Human

Immunogène

Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged).

Hôte

Mouse

Clonalité

Monoclonal

Clone

4F9

Isotype

IgG2b

Conjuguer

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Masse moléculaire

~57 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

58 kDa glucose regulated protein, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER 60, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, ER p57, ER protein 57, ER protein 60, ERp 57, ERp60, ERp61, Glucose Regulated Protein 58 Kd, GRP 57, GRP 58, GRP57, HsT17083, p58, PDIA 3, PDIA3, PDIA3_HUMAN, Phospholipase C alpha, PI PLC, Protein disulfide isomerase A3, Protein disulfide isomerase family A member 3, Protein disulfide-isomerase A3

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (2)

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Figure 1: Western Blot - Anti-ERp57 Antibody [4F9] (A304860)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of 57 kDa Erp57 protein using Anti-ERp57 Antibody [4F9] (A304860) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 57 kDa.

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Figure 2: Immunofluorescence - Anti-ERp57 Antibody [4F9] (A304860)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ERp57 Antibody [4F9] (A304860), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ERp57 Antibody. (D) Composite.