Anti-ERK1 Anticorps (A83709)

Name: Anti-ERK1 Antibody
Brand: Antibodies.com
SKU: A83709
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Novus (NB100-1508). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre ERK1 pour ELISA, WB, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-ERK1 Antibody

Description du produit

Goat polyclonal antibody to ERK1.

Applications

ELISA, WB, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:4,000, WB: 0.1-1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Réactivité croisée

This antibody is not expected to cross react with ERK2

Immunogène

Synthetic peptide corresponding to the N terminal region of human ERK1.

Séquence

GGEPRRTEGVGP-C

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

40 kDa

MW prédit

40.1kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

ERK, ERK 1, ERK-1, ERT 2, ERT2, Extracellular Signal Regulated Kinase 1, Extracellular signal related kinase, Extracellular signal related kinase 1, Extracellular signal-regulated kinase 1, HGNC6877, HS44KDAP, HUMKER1A, Insulin Stimulated MAP2 Kinase, Insulin-stimulated MAP2 kinase, MAP Kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK, MAPK 1, MAPK 3, MAPK1, Mapk3, MGC20180, Microtubule Associated Protein 2 Kinase, Microtubule-associated protein 2 kinase, Mitogen Activated Protein Kinase 3, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, MK03_HUMAN, OTTHUMP00000174538, OTTHUMP00000174541, p44 ERK1, p44 MAPK, p44-ERK1, p44-MAPK, P44ERK1, P44MAPK, PRKM 3, PRKM3, Protein Kinase Mitogen Activated 3

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

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Figure 1: Western Blot - Anti-ERK1 Antibody (A83709)

ERK1 expression in Human Cerebellum (A) and Frontal Cortex (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ERK1 Antibody (A83709) at 0.1µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-ERK1 Antibody (A83709)

ERK1 expression in A431 (A) and HepG2 (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody was performed with Anti-ERK1 Antibody (A83709) at 1µg/ml (A) or 0.3µg/ml (B) and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-ERK1 Antibody (A83709)

ERK1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ERK1 Antibody (A83709) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-ERK1 Antibody (A83709)

ERK1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ERK1 Antibody (A83709) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.