ERAB Cell Based Kit Elisa (A103232)

Nom du produit
ERAB Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This ERAB Cell Based ELISA Kit allows for the detection of ERAB and the effects that certain stimulation conditions have on ERAB expression in different cell lines. Qualitative determination of ERAB concentration is achieved by an indirect ELISA format. In essence, the ERAB is captured by Anti-ERAB Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this ERAB Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
17 beta hydroxysteroid dehydrogenase 10, 17 beta hydroxysteroid dehydrogenase type 10, 17-beta-HSD 10, 17-beta-hydroxysteroid dehydrogenase 10, 17b HSD10, 3 hydroxy 2 methylbutyryl CoA dehydrogenase, 3 hydroxyacyl CoA dehydrogenase type 2, 3 hydroxyacyl CoA dehydrogenase type II, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, 3-hydroxyacyl-CoA dehydrogenase type II, 3-hydroxyacyl-CoA dehydrogenase type-2, AB binding alcohol dehydrogenase, ABAD, Ads9, Amyloid beta binding polypeptide, Amyloid beta peptide binding alcohol dehydrogenase, Amyloid beta peptide binding protein, CAMR, DUPXp11.22, Endoplasmic Reticulum Amyloid Binding Protein, Endoplasmic reticulum associated amyloid beta peptide binding protein, Endoplasmic reticulum-associated amyloid beta-peptide-binding protein, ER associated amyloid beta-binding protein, HADH 2, HADH2, HCD 2, HCD2, HCD2_HUMAN, Hsd17b10, Hydroxyacyl CoA Dehydrogenase type II, Hydroxyacyl Coenzyme A dehydrogenase type II, Hydroxysteroid (17 beta) dehydrogenase 10, Mental retardation X linked syndromic 11, MHBD, Mitochondrial L3 Hydroxyacyl CoA Dehydrogenase, Mitochondrial ribonuclease P protein 2, Mitochondrial RNase P protein 2, MRPP2, MRX17, SCHAD, SDR5C1, Short chain dehydrogenase/reductase family 5C member 1, Short chain L 3 hydroxyacyl CoA dehydrogenase type 2, Short chain type dehydrogenase/reductase XH98G2, Short-chain type dehydrogenase/reductase XH98G2, Type 10 17b HSD, Type 10 17beta hydroxysteroid dehydrogenase, Type II HADH, XH98G2
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
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