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EPHA7 Cell Based Kit Elisa (A103089)

Nom du produit
EPHA7 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This EPHA7 Cell Based ELISA Kit allows for the detection of EPHA7 and the effects that certain stimulation conditions have on EPHA7 expression in different cell lines. Qualitative determination of EPHA7 concentration is achieved by an indirect ELISA format. In essence, the EPHA7 is captured by Anti-EPHA7 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this EPHA7 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
Cek 11, Developmental kinase 1, EBK, EHK-3, EHK3, EK11, Embryonic brain kinase, EPH homology kinase 3, Eph receptor A7, EPH-like kinase 11, EPHA7_HUMAN, Ephrin receptor Eph A7, Ephrin type A receptor 7, Ephrin type-A receptor 7, hEK11, MDK 1, Receptor protein tyrosine kinase HEK 11, Tyrosine protein kinase receptor EHK 3
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - EPHA7 Cell Based ELISA Kit (CB5237) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - EPHA7 Cell Based ELISA Kit (CB5237)
Western blot analysis of extracts from Jurkat cells/COLO205 cells, using Anti-EPHA7 Antibody.
Protocol Diagram - EPHA7 Cell Based ELISA Kit (CB5237) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: EPHA7 Cell Based ELISA Kit (CB5237)
A diagram showing the protocol for EPHA7 Cell Based ELISA Kit.
Protocol Summary - EPHA7 Cell Based ELISA Kit (CB5237) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: EPHA7 Cell Based ELISA Kit (CB5237)
A summary of the protocol for EPHA7 Cell Based ELISA Kit.
Western Blot - Control Antibody - EPHA7 Cell Based ELISA Kit (CB5237) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - EPHA7 Cell Based ELISA Kit (CB5237)
The mouse monoclonal antibody to GAPDH is used as a positive control inEPHA7 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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