The antibody detects endogenous levels of EPHA2 / 3 / 4 only when phosphorylated at tyrosine 588 / 596
Applications
WB, IF
Recommander des dilutions
Western blotting: 1:500~1:1000 Immunofluorescence: 1:100~1:200
Reactivité
Human, Mouse
Immunogène
Peptide sequence around phosphorylation site of tyrosine 588 / 596 (K-T-Y(p)-V-R) / (R-T-Y(p)-V-D) derived from Human EPHA2 / 3 / 4.
Hôte
Rabbit
Clonalité
Polyclonal
Conjuguer
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody #11690.The lane on the right is treated with the antigen-specific peptide.
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