Anti-eIF4G1 Antibody (A10162)

Name: Anti-eIF4G1 Antibody
Brand: Antibodies.com
SKU: A10162
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to eIF4G1 for WB, IHC and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-eIF4G1 Antibody

Description

Rabbit polyclonal antibody to eIF4G1.

Applications

WB, IHC, IP

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, IP: 1:100-1:500

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_886553.3).

Sequence

PEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

220 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

eIF-4-gamma 1, eIF-4G 1, eIF-4G1, EIF4F, EIF4G, EIF4GI, Eukaryotic translation initiation factor 4 gamma 1, p220

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-eIF4G1 Antibody (A10162)

Western blot analysis of extracts of HeLa cells, using Anti-eIF4G1 Antibody (A10162) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-eIF4G1 Antibody (A10162)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-eIF4G1 Antibody (A10162) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Western Blot - Anti-eIF4G1 Antibody (A10162)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-eIF4G1 Antibody (A10162). This Western blot was performed on the immunoprecipitate using Anti-eIF4G1 Antibody (A10162) at a dilution of 1:500.

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