Anti-eIF4EBP1 (phospho Thr70) Anticorps [ARC53785] (A308921)

Name: Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785]
Brand: Antibodies.com
SKU: A308921
Price: 540.0 USD
Availability: InStock

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$540

Anticorps monoclonal [ARC53785] de lapin contre eIF4EBP1 (phospho Thr70) pour WB.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785]

Description du produit

Rabbit monoclonal [ARC53785] antibody to eIF4EBP1 (phospho Thr70).

Applications

Recommander des dilutions

WB: 1:2,000-1:6,000

Reactivité

Human, Mouse

Immunogène

A synthetic phosphorylated peptide around T70 of human Phospho-eIF4EBP1-T70 (NP_004086.1).

Séquence

TKTPP

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC53785

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

15 - 20 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (2)

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Figure 1: Western Blot - Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785] (A308921)

Western blot analysis of extracts of various cell lines, using Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785] (A308921) at 1:5,000 dilution. 293T cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785] (A308921)

Western blot analysis of extracts of NIH/3T3, using Anti-eIF4EBP1 (phospho Thr70) Antibody [ARC53785] (A308921) at 1:5,000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.