Rabbit polyclonal antibody to eIF4EBP1 (phospho Thr45).
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:100
Reactivity
Human
Immunogen
A phospho specific peptide corresponding to residues surrounding T45 of human Phospho-eIF4EBP1-T45.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
18 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
4E-BP1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Western Blot - Anti-eIF4EBP1 (phospho Thr45) Antibody (A10899)
Western blot analysis of extracts from HeLa cells using Anti-eIF4EBP1 (phospho Thr45) Antibody (A10899). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
