Rabbit polyclonal antibody to eIF4EBP1 (phospho Ser65).
Applications
WB, IP
Recommander des dilutions
WB: 1:500-1:1,000, IP: 1:50-1:100
Reactivité
Human
Immunogène
A synthetic phosphorylated peptide around S65 of human Phospho-eIF4EBP1-S65 (NP_004086.1).
Séquence
RNSPV
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
Unconjugated
Purification
Affinity purification.
Masse moléculaire
20 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Figure 1: Western Blot - Anti-eIF4EBP1 (phospho Ser65) Antibody (A16404)
Western blot analysis of extracts of 293T cells, using Anti-eIF4EBP1 (phospho Ser65) Antibody (A16404) at 1:1,000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Produits alternatifs à Anti-eIF4EBP1 (phospho Ser65) Anticorps (A16404)