Anti-eIF4E Anticorps (A309356)

Name: Anti-eIF4E Antibody
Brand: Antibodies.com
SKU: A309356
Price: 470.0 USD
Availability: InStock

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$470

Anticorps polyclonal de lapin contre eIF4E pour WB, IHC et ICC/IF.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-eIF4E Antibody

Description du produit

Rabbit polyclonal antibody to eIF4E.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200, IHC: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant protein of human eIF4E.

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

29 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

AUTS19, CBP, eIF 4E, EIF 4F, eIF 4F 25 kDa subunit, eIF-4E, eIF-4F 25 kDa subunit, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4 E, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4E like 1, IF4E_HUMAN, Messanger RNA Cap Binding Protein eIF 4E, MGC111573, mRNA cap binding protein, mRNA cap-binding protein

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (9)

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Figure 1: Western Blot - Anti-eIF4E Antibody (A309356)

Western blot analysis of various lysates, using Anti-eIF4E Antibody (A309356) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-eIF4E Antibody (A309356)

Western blot analysis of extracts from wild type(WT) and eIF4E knockdown (KD) 293T(KD) cells, using Anti-eIF4E Antibody (A309356) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-eIF4E Antibody (A309356)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-eIF4E Antibody (A309356) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-eIF4E Antibody (A309356)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-eIF4E Antibody (A309356) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.