Figure 1: Western Blot - Anti-eIF4B Antibody (A91348)
Western blot analysis of extracts of Rat skeletal muscle, using Anti-eIF4B Antibody (A91348) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.
Figure 2: Western Blot - Anti-eIF4B Antibody (A91348)
Western blot analysis of extracts of Mouse kidney, using Anti-eIF4B Antibody (A91348) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of A-549 using Anti-eIF4B Antibody (A91348) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 using Anti-eIF4B Antibody (A91348) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of HeLa using Anti-eIF4B Antibody (A91348) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).
