Western blot analysis of various lysates, using Anti-eIF4A1 Antibody (A14643) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence analysis of C6 cells using Anti-eIF4A1 Antibody (A14643) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-eIF4A1 Antibody (A14643) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-eIF4A1 Antibody (A14643) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-eIF4A1 Antibody (A14643). This Western blot was performed on the immunoprecipitate using Anti-eIF4A1 Antibody (A14643) at a dilution of 1:1000.
