Anti-EIF2S1 (phospho Ser51) Anticorps (A89604)

Name: Anti-EIF2S1 (phospho Ser51) Antibody
Brand: Antibodies.com
SKU: A89604
Price: 435.0 USD
Availability: InStock

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$435

Anticorps polyclonal de lapin contre EIF2S1 (phospho Ser51) pour WB, ICC/IF et IP.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

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Mots clés :

Ribosomal Marker

Spécifications

Nom du produit

Anti-EIF2S1 (phospho Ser51) Antibody

Description du produit

Rabbit polyclonal antibody to EIF2S1 (phospho Ser51).

Applications

WB, ICC/IF, IP

Recommander des dilutions

WB: 1:1,000-1:2,000, IP: 1:50-1:100, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around S51 of human eIF2a (NP_004085.1).

Séquence

SELSR

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

38 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

EIF 2, EIF 2 alpha, EIF 2A, EIF 2alpha, eIF-2-alpha, eIF-2A, EIF-2alpha, EIF2, EIF2 alpha, EIF2A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit 1 alpha, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, Eukaryotic translation initiation factor 2 subunit alpha, IF2A_HUMAN

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-EIF2S1 (phospho Ser51) Antibody (A89604)

Western blot analysis of extracts of HeLa cells, using Anti-EIF2S1 (phospho Ser51) Antibody (A89604) at 1:2,000 dilution or EIF2S1 antibody (A0764). HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunofluorescence - Anti-EIF2S1 (phospho Ser51) Antibody (A89604)

Immunofluorescence analysis of HeLa and HeLa treated with CA cells using Anti-EIF2S1 (phospho Ser51) Antibody (A89604) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Western Blot - Anti-EIF2S1 (phospho Ser51) Antibody (A89604)

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-EIF2S1 (phospho Ser51) Antibody (A89604). This Western blot was performed on the immunoprecipitate using Anti-EIF2S1 (phospho Ser51) Antibody (A89604) at a dilution of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.