Anti-EEF2K (phospho Ser366) Anticorps (A87764)

Name: Anti-EEF2K (phospho Ser366) Antibody
Brand: Antibodies.com
SKU: A87764
Price: 435.0 USD
Availability: InStock

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$435

Anticorps polyclonal de lapin contre EEF2K (phospho Ser366) pour WB.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-EEF2K (phospho Ser366) Antibody

Description du produit

Rabbit polyclonal antibody to EEF2K (phospho Ser366).

Applications

Recommander des dilutions

WB: 1:500-1:2,000

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around S366 of human EEF2K (NP_037434.1).

Séquence

TLSGS

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

110 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

C86191, Calcium/calmodulin dependent eukaryotic elongation factor 2, Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, Calmodulin dependent protein kinase III, cb365, EC 2.7.11.20, eEF 2 kinase, eEF 2K, eEF-2 kinase, eEF-2K, EEF2K protein, EF2K_HUMAN, Elongation factor 2 kinase, Eukaryotic elongation factor 2 kinase, fa04b08, HSU93850, kinase eEF2K, MGC45041, SMEF2K, wu:fa04b08, zgc:100973

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-EEF2K (phospho Ser366) Antibody (A87764)

Western blot analysis of extracts of HeLa cells, using Anti-EEF2K (phospho Ser366) Antibody (A87764) at 1:1,000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 15s.

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Figure 2: Western Blot - Anti-EEF2K (phospho Ser366) Antibody (A87764)

Western blot analysis of extracts of various cell lines, using Anti-EEF2K (phospho Ser366) Antibody (A87764) at 1:1,000 dilution. NIH/3T3 and C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour or PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-EEF2K (phospho Ser366) Antibody (A87764)

Western blot analysis of extracts of HeLa cells, using Anti-EEF2K (phospho Ser366) Antibody (A87764) at 1:1,000 dilution or EEF2K antibody (A5404). Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 15s.