EdU Cell Proliferation Image Kit (Green Fluorescence) (A319642)

$205
Frais de livraison
Date de livraison
Livraison sous 7-8 jours ouvrables.
Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com
Nom du produit
EdU Cell Proliferation Image Kit (Green Fluorescence)
Applications
Fluorescence Microscopy
Principe du test
This kit is a novel alternative to the BrdU assay. EdU (5-ethynyl-2´-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. Comparing to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation (typically using HCl or heat or digestion with DNase) for detection of the incorporated nucleoside. Detection is based on a click reaction, a copper-catalyzed covalent reaction between an azide and an alkyne, and is complete within 30 minutes.
Composants
EdU (10mM), BSA Wash Solution (5X), Fluor 488 azide, Reaction Buffer (10X), Copper Reagent, Reducing Agent, Hoechst 33342 (1000X), Hydroxyurea
Maxima d'absorption
501 nm
Maxima d'émission
525 nm
Stockage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
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