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EDD Cell Based Kit Elisa (A103565)

Nom du produit
EDD Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This EDD Cell Based ELISA Kit allows for the detection of EDD and the effects that certain stimulation conditions have on EDD expression in different cell lines. Qualitative determination of EDD concentration is achieved by an indirect ELISA format. In essence, the EDD is captured by Anti-EDD Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this EDD Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
4432411E13Rik, AW549941, C77315, D030042K14, DD5, E3 identified by differential display, E3 ubiquitin protein ligase, HECT domain containing, 1, E3 ubiquitin-protein ligase, E3 ubiquitin-protein ligase UBR5, EDD 1, EDD1, FLJ11310, HECT domain-containing 1, hHYD, HYD, Hyperplastic discs protein homolog, Hyperplastic discs, Drosophila, homolog of, KIAA0896, MGC57263, mKIAA0896, Progestin induced protein, Progestin-induced protein, Rat100, Ubiquitin protein ligase, Ubiquitin protein ligase E3 component n recognin 5, UBR5, UBR5_HUMAN
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - EDD Cell Based ELISA Kit (CB5219) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - EDD Cell Based ELISA Kit (CB5219)
Western blot analysis of the lysates from K562 cells using Anti-EDD antibody.
Protocol Diagram - EDD Cell Based ELISA Kit (CB5219) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: EDD Cell Based ELISA Kit (CB5219)
A diagram showing the protocol for EDD Cell Based ELISA Kit.
Protocol Summary - EDD Cell Based ELISA Kit (CB5219) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: EDD Cell Based ELISA Kit (CB5219)
A summary of the protocol for EDD Cell Based ELISA Kit.
Western Blot - Control Antibody - EDD Cell Based ELISA Kit (CB5219) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - EDD Cell Based ELISA Kit (CB5219)
The mouse monoclonal antibody to GAPDH is used as a positive control inEDD Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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