This antibody recognises the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus or at an internal site on target proteins.Rat anti DYKDDDDK Tag antibody, clone 6F7 works at neutral pH and has very low background staining in immunocytochemistry.
Applications
Flow Cytometry, WB, ELISA, IHC-P, IP, IF
Dilutions
WB: 1:1,000
Reactivity
Species Independent
Host
Rat
Clonality
Monoclonal
Clone ID
6F7
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti DYKDDDDK Tag antibody, clone 6F7 recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus or at an internal site on target proteins.Rat anti DYKDDDDK Tag antibody, clone 6F7 works at neutral pH and has very low background staining in immunocytochemistry.
Synonyms
DDDDK epitope tag, DDDDK tag, DDDK, ddk, DYKDDDDK, DYKDDDDK epitope tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG, FLAG tag
