Figure 1: Western Blot - Anti-Dscam Antibody (A83347)
Dscam expression in HepG2 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Dscam Antibody (A83347) at 2µg/ml and detected by chemiluminescence.
Dscam expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Dscam Antibody (A83347) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Dscam expression in MCF7 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Dscam Antibody (A83347) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
