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Anti-DNA polymerase eta Anticorps [5H10] (A358)

Identification of Pol eta in whole cell extract of HeLa cells by western blot with Anti-DNA polymerase eta Antibody. Lane 1: Recombinant full-size Pol eta with His6 tag at C-teminus (Positive Control). Lane 2: Whole cell extract of HeLa cells (40µg) (Positive Control). Lane 3: Whole cell extract of XP2SA (XP-V) cells (40µg) (Negative Control). Pol eta is detected at ~80 kDa position. 8% gel was used. Immunofluorescence staining of DNA polymerase eta in HeLa cells with Anti-DNA polymerase eta Antibody. HeLa cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in PBS for 10 min. Anti-DNA polymerase eta Antibody was used at 1:1,000 dilution.
$535

Anticorps monoclonal [5H10] de souris contre DNA polymerase eta pour WB et IF.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 4-6 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-DNA polymerase eta Antibody [5H10]
Description du produit
Mouse monoclonal (5H10) antibody to DNA polymerase eta.
Applications
WB, IF
Recommander des dilutions
WB: 1:500-1:2,000, IF: 1:200-1:1,000
Reactivité
Human
Immunogène
Recombinant full length human Pol eta protein (His tag).
Hôte
Mouse
Clonalité
Monoclonal
Clone
5H10
Isotype
IgG1
Conjuguer

Unconjugated

Purification
Ion exchange chromatography.
Concentration
1 mg/ml
Pureté
> 90% (by SDS-PAGE)
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
FLJ16395, FLJ21978, Polh, POLH_HUMAN, polymerase DNA directed eta, RAD30, RAD30 homolog A, RAD30A, Xeroderma pigmentosum variant type protein, XP V, XPV
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Identification of Pol eta in whole cell extract of HeLa cells by western blot with Anti-DNA polymerase eta Antibody. Lane 1: Recombinant full-size Pol eta with His6 tag at C-teminus (Positive Control). Lane 2: Whole cell extract of HeLa cells (40µg) (Positive Control). Lane 3: Whole cell extract of XP2SA (XP-V) cells (40µg) (Negative Control). Pol eta is detected at ~80 kDa position. 8% gel was used.Cliquez pour ouvrir le point de vue élargi
Figure 1: Anti-DNA polymerase eta Antibody [5H10] (70-071)
Anti-DNA polymerase eta Antibody
Immunofluorescence staining of DNA polymerase eta in HeLa cells with Anti-DNA polymerase eta Antibody. HeLa cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in PBS for 10 min. Anti-DNA polymerase eta Antibody was used at 1:1,000 dilution.Cliquez pour ouvrir le point de vue élargi
Figure 2: Immunofluorescence - Anti-DNA polymerase eta Antibody [5H10] (70-071)
Anti-DNA polymerase eta Antibody

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