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Anti-DNA polymerase eta Antibody [5H10] (A358)

Identification of Pol eta in whole cell extract of HeLa cells by western blot with Anti-DNA polymerase eta Antibody. Lane 1: Recombinant full-size Pol eta with His6 tag at C-teminus (Positive Control). Lane 2: Whole cell extract of HeLa cells (40µg) (Positive Control). Lane 3: Whole cell extract of XP2SA (XP-V) cells (40µg) (Negative Control). Pol eta is detected at ~80 kDa position. 8% gel was used. Immunofluorescence staining of DNA polymerase eta in HeLa cells with Anti-DNA polymerase eta Antibody. HeLa cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in PBS for 10 min. Anti-DNA polymerase eta Antibody was used at 1:1,000 dilution.
$555

Mouse monoclonal (5H10) antibody to DNA polymerase eta for WB and IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-DNA polymerase eta Antibody [5H10]
Description
Mouse monoclonal (5H10) antibody to DNA polymerase eta.
Applications
WB, IF
Dilutions
WB: 1:500-1:2,000, IF: 1:200-1:1,000
Reactivity
Human
Immunogen
Recombinant full length human Pol eta protein (His tag).
Host
Mouse
Clonality
Monoclonal
Clone ID
5H10
Isotype
IgG1
Conjugate

Unconjugated

Purification
Ion exchange chromatography.
Concentration
1 mg/ml
Purity
> 90% (by SDS-PAGE)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
POLH, RAD30, RAD30 homolog A, RAD30A, Xeroderma pigmentosum variant type protein, XPV
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Identification of Pol eta in whole cell extract of HeLa cells by western blot with Anti-DNA polymerase eta Antibody. Lane 1: Recombinant full-size Pol eta with His6 tag at C-teminus (Positive Control). Lane 2: Whole cell extract of HeLa cells (40µg) (Positive Control). Lane 3: Whole cell extract of XP2SA (XP-V) cells (40µg) (Negative Control). Pol eta is detected at ~80 kDa position. 8% gel was used.Enlarge Image

Anti-DNA polymerase eta Antibody [5H10] (A358)

Anti-DNA polymerase eta Antibody
Immunofluorescence staining of DNA polymerase eta in HeLa cells with Anti-DNA polymerase eta Antibody. HeLa cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in PBS for 10 min. Anti-DNA polymerase eta Antibody was used at 1:1,000 dilution.Enlarge Image

Immunofluorescence - Anti-DNA polymerase eta Antibody [5H10] (A358)

Anti-DNA polymerase eta Antibody
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