Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
DNA directed DNA polymerase beta, DNA pol beta, DNA polymerase beta subunit, DPOLB_HUMAN, MGC125976, Pol B, Pol beta, POLB, Polymerase (DNA directed) beta
Figure 1: Western Blot - Anti-DNA Polymerase beta Antibody (A13592)
Western blot analysis of extracts of various cell lines, using Anti-DNA Polymerase beta Antibody (A13592) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Figure 2: Western Blot - Anti-DNA Polymerase beta Antibody (A13592)
Western blot analysis of extracts from normal (control) and DNA Polymerase beta knockout (KO) HeLa cells, using Anti-DNA Polymerase beta Antibody (A13592) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-DNA Polymerase beta Antibody (A13592) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-DNA Polymerase beta Antibody (A13592) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
![Western Blot - Anti-DNA Polymerase beta Antibody [ARC0752] (A307402) - Antibodies.com](https://cdn.antibodies.com/image/catalog/307/A307402_1.jpg?profile=product_alternative)
