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DNA Polymerase alpha Cell Based Kit Elisa (A103593)

Nom du produit
DNA Polymerase alpha Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This DNA Polymerase alpha Cell Based ELISA Kit allows for the detection of DNA Polymerase alpha and the effects that certain stimulation conditions have on DNA Polymerase alpha expression in different cell lines. Qualitative determination of DNA Polymerase alpha concentration is achieved by an indirect ELISA format. In essence, the DNA Polymerase alpha is captured by Anti-DNA Polymerase alpha Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this DNA Polymerase alpha Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
DKFZp686K1672, DNA polymerase alpha 1 catalytic subunit, DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180, DNA polymerase alpha p180 subunit, DPOLA_HUMAN, OTTHUMP00000023089, OTTHUMP00000023090, P180, p180 subunit, POLA, POLA1, Polymerase (DNA directed), alpha 1, catalytic subunit, Polymerase (DNA-directed), alpha (70kD)
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - DNA Polymerase alpha Cell Based ELISA Kit (CB5835) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - DNA Polymerase alpha Cell Based ELISA Kit (CB5835)
Western blot analysis of extracts from HepG2/293/Jurkat cells, using Anti-DNA Polymerase alpha Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - DNA Polymerase alpha Cell Based ELISA Kit (CB5835) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: DNA Polymerase alpha Cell Based ELISA Kit (CB5835)
A diagram showing the protocol for DNA Polymerase alpha Cell Based ELISA Kit.
Protocol Summary - DNA Polymerase alpha Cell Based ELISA Kit (CB5835) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: DNA Polymerase alpha Cell Based ELISA Kit (CB5835)
A summary of the protocol for DNA Polymerase alpha Cell Based ELISA Kit.
Western Blot - Control Antibody - DNA Polymerase alpha Cell Based ELISA Kit (CB5835) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - DNA Polymerase alpha Cell Based ELISA Kit (CB5835)
The mouse monoclonal antibody to GAPDH is used as a positive control inDNA Polymerase alpha Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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