Anti-DKK1 Anticorps (A84041)

Name: Anti-DKK1 Antibody
Brand: Antibodies.com
SKU: A84041
Price: 480.0 USD
Availability: InStock

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$480

Anticorps polyclonal de chèvre contre DKK1 pour ELISA, WB, IF, IHC et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-DKK1 Antibody

Description du produit

Goat polyclonal antibody to DKK1.

Applications

ELISA, WB, IF, IHC, Flow Cytometry

Recommander des dilutions

ELISA: 1:128,000, WB: 1-3µg/ml, IHC: 10µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the C terminal region of human DKK1.

Séquence

CDHHQASNSSRLHT

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

38 kDa

MW prédit

28.7kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

Dickkopf 1, Dickkopf 1 homolog, Dickkopf 1 like, Dickkopf homolog 1 , Dickkopf like protein 1, Dickkopf related protein 1, Dickkopf WNT signaling pathway inhibitor 1, Dickkopf-1, Dickkopf-related protein 1, DKK 1, Dkk-1, DKK1_HUMAN, hDkk 1 , hDkk-1, hDkk1 , mDkk1, SK

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-DKK1 Antibody (A84041)

DKK1 expression in MCF7 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-DKK1 Antibody (A84041) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-DKK1 Antibody (A84041)

DKK1 expression in Human Placenta analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-DKK1 Antibody (A84041) at 10µg/ml and revealed with alkaline phosphatase (AP).

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Figure 3: Immunofluorescence - Anti-DKK1 Antibody (A84041)

DKK1 expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-DKK1 Antibody (A84041) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-DKK1 Antibody (A84041)

DKK1 expression in HepG2 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-DKK1 Antibody (A84041) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.