Anti-Dicer Anticorps [S167-7] (A304979)

Name: Anti-Dicer Antibody [S167-7]
Brand: Antibodies.com
SKU: A304979
Price: 500.0 USD
Availability: InStock

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$500

Anticorps monoclonal [S167-7] de souris contre Dicer pour WB et ICC/IF.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-Dicer Antibody [S167-7]

Description du produit

Mouse monoclonal [S167-7] antibody to Dicer.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivité

Human, Mouse, Rat

Immunogène

Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer.

Hôte

Mouse

Clonalité

Monoclonal

Clone

S167-7

Isotype

IgG1

Conjuguer

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Masse moléculaire

~215 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

DCR, DCR1, Dicer 1 ribonuclease III, Dicer 1 ribonuclease type III, DICER1, DICER_HUMAN, Double-strand-specific ribonuclease, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase motif, HERNA, KIAA0928, MNG1, RMSE2

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-Dicer Antibody [S167-7] (A304979)

Western blot analysis of rat brain membrane showing detection of ~215 kDa Dicer protein using Anti-Dicer Antibody [S167-7] (A304979) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: rat brain Membrane. Load: 10 µg. Block: 5% milk. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at room temperature. Color Development: TMB solution for 10 min at room temperature. Predicted/Observed Size: ~215 kDa.

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Figure 2: Immunofluorescence - Anti-Dicer Antibody [S167-7] (A304979)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Dicer Antibody [S167-7] (A304979), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5,000 for 60 minutes room temperature, 5 minutes room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Dicer Antibody. (D) Composite.

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Figure 3: Immunofluorescence - Anti-Dicer Antibody [S167-7] (A304979)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-Dicer Antibody [S167-7] (A304979), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Dicer Antibody (D) Composite.