Anti-DARPP-32 (phospho Thr75) Antibody (A93772)

Name: Anti-DARPP-32 (phospho Thr75) Antibody
Brand: Antibodies.com
SKU: A93772
Price: 190.0 USD
Availability: InStock

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$190

Rabbit polyclonal antibody to DARPP-32 (phospho Thr75) for WB, IHC and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-DARPP-32 (phospho Thr75) Antibody

Description

Rabbit polyclonal antibody to DARPP-32 (phospho Thr75).

Specificity

This antibody detects endogenous levels of DARPP-32 only when phosphorylated at Thr75.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:1000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human DARPP-32 around the phosphorylation site of Thr75 (amino acids 41-90).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

22kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-DARPP-32 (phospho Thr75) Antibody (A93772)

Western blot analysis of lysates from COS7 cells treated with Forskolin 40nM 30' using Anti-DARPP-32 (phospho Thr75) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Immunohistochemistry - Anti-DARPP-32 (phospho Thr75) Antibody (A93772)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-DARPP-32 (phospho Thr75) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Western Blot - Anti-DARPP-32 (phospho Thr75) Antibody (A93772)

Western blot analysis of various cells using Anti-DARPP-32 (phospho Thr75) Antibody.

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Immunohistochemistry - Anti-DARPP-32 (phospho Thr75) Antibody (A93772)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-DARPP-32 (phospho Thr75) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.